Journal: Journal of Bacteriology

Article Title: FleQ finetunes the expression of a subset of BrlR-activated genes to enable antibiotic tolerance by Pseudomonas aeruginosa biofilms

doi: 10.1128/jb.00503-24

Figure Lengend Snippet: FleQ contributes to biofilm formation in a manner independent of SagS and SagS downstream signaling. ( A ) Representative confocal images of 3-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS . White bar = 100 µm. ( B ) Quantitative of the biofilm biomass and biofilm height by COMSTAT of biofilms formed by P. aeruginosa PAO1, Δ fleQ, and double mutant Δ fleQΔsagS. * *, ***, significantly different ( P = 0.0001, <0.0001, respectively) relative to PAO1 using ANOVA followed by the Bartlett’s test. Ns, not significant. ( C ) Total cellular c-di-GMP levels in 6-day-old biofilms formed by P. aeruginosa PAO1, Δ fleQ, and ΔsagS, as determined by HPLC quantitative analysis, followed by normalization relative to total cell protein content. *, P < 0.05, relative to PAO1 using ANOVA followed by Dunnett’s T3 multiple comparisons test. ( D ) Representative confocal images of 6-day-old P. aeruginosa Δ fleQ and ΔsagS biofilms and mutant biofilms expressing bfiR and gcbA. gcbA encodes diguanylate cyclase GcbA, bfiR the two-component response regulator BfiR. White bar = 100 µm. All experiments were performed in triplicate. Error bars indicate standard deviations.

Article Snippet: Quantitative analysis of the images was performed using COMSTAT MATLAB package and COMSTAT2 ( , ).

Techniques: Mutagenesis, Expressing

Journal: BMC Microbiology

Article Title: Marine bacteria from the French Atlantic coast displaying high forming-biofilm abilities and different biofilm 3D architectures

doi: 10.1186/s12866-015-0568-4

Figure Lengend Snippet: COMSTAT analyses of biofilms formed on glass surfaces, after 24 h of growth under dynamic conditions. A , B , C : bacterial isolates from mudflat biofilms (white bars). D : bacterial isolate from corrosion product-microorganism composite biofilms (black bars). Significant differences were only observed in isolate pairs A-B , A-B , A–D for maximal thickness, A – C for average thickness and are indicated by * ( p < 0.05) or ** ( p < 0.01) on the upper part of the Figure. In all the other cases, the differences were not significant

Article Snippet: The biofilm stacks were analysed with the COMSTAT software (developed in MATLAB, [ ]) to estimate the maximal and average thicknesses (μm) and the biovolume (μm 3 μm -2 ).

Techniques:

Journal: Scientific Reports

Article Title: Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells

doi: 10.1038/s41598-019-52115-7

Figure Lengend Snippet: Compositional analysis and ultrastructural differences in multi-species oral biofilms. Bacterial DNA was extracted from multi- species biofilms using DNeasy Qiagen kit for quantification of each species using SYBR GreenER based quantitative PCR ( A – C ). Unique biofilm morphology and architecture was visualised using scanning electron microscopy (( D – F ); lower magnification ×500, and higher magnification ×5000 inset) and transmission electron microscopy (( G – I ); lower magnification ×15000, and higher magnification ×25000 inset). Confocal stacked images representative of biofilms stained with SYTO9 and PI to show live and dead cells as viewed using the Zeiss LSM 780 confocal laser scanning microscope. Confocal images were taken at ×40 magnification and image stacks compiled using COMSTAT2 program. Data for qPCR compositional analysis of biofilms representative of mean +/− SEM for n = 3 from two individual experiments. Abbreviations; A. a* = Aggregatibacter actinomycetemcomitans .

Article Snippet: Image were taken at x 40 magnification and image stacks compiled using COMSTAT2 program (MATLAB; MA, USA).

Techniques: Real-time Polymerase Chain Reaction, Electron Microscopy, Transmission Assay, Staining, Laser-Scanning Microscopy